IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T
cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type
mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and
CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 is a 75.0
kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503
total amino acid residues).
The BioGems Human IL-12(p70) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase
immunoassay specially designed to measure Human IL-12(p70) with a 96-well strip plate that is pre-coated
with mouse monoclonal antibody specific for IL-12(p70). It contains E. coli-expressed recombinant Human IL-
12(p70). The detection antibody is a biotinylated goat polyclonal antibody specific for IL-12(p70). The kit is
analytically validated with ready to use reagents.