PPM1A (C-term) Peptide
产品名称： PPM1A (C-term) Peptide
英文名称： PPM1A (C-term) Peptide
产品编号： P3311
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of PPM1A.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PPM1A.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Protein phosphatase-2C alpha as a positive regulator of insulin sensitivity through direct activation of phosphatidylinositol 3-kinase in 3T3-L1 adipocytes.
Yoshizaki T, Maegawa H, Egawa K, Ugi S, Nishio Y, Imamura T, Kobayashi T, Tamura S, Olefsky JM, Kashiwagi A.J Biol Chem. 2004 May 21;279(21):22715-26. Epub 2004 Mar 11.
- 2.
- Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3.
Flajolet M, Rakhilin S, Wang H, Starkova N, Nuangchamnong N, Nairn AC, Greengard P.Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):16006-11. Epub 2003 Dec 8.
- 3.
- Cell cycle regulation and p53 activation by protein phosphatase 2C alpha.
Ofek P, Ben-Meir D, Kariv-Inbal Z, Oren M, Lavi S.J Biol Chem. 2003 Apr 18;278(16):14299-305. Epub 2003 Jan 3.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of PPM1A polyclonal antibody (Cat # PAB4156) in mouse kidney tissue lysate (35 ug/lane). PPM1A (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PPM1A polyclonal antibody (Cat # PAB4156) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PPM1A polyclonal antibody (Cat # PAB4156) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 5494
- Protein Accession#:
- NP_066283;P35813
- Gene Name:
- PPM1A
- Gene Alias:
- FLJ42306,MGC9201,PP2C-ALPHA,PP2CA
- Gene Description:
- protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
- Omim ID:
- 606108
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
- Other Designations:
- protein phosphatase 1A,protein phosphatase 2C alpha isoform
- Gene Pathway
- Related Disease