FITC标记的细胞周期后期促进蛋白亚基10抗体-抗体-抗体-生物在线
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FITC标记的细胞周期后期促进蛋白亚基10抗体

FITC标记的细胞周期后期促进蛋白亚基10抗体

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产品名称： FITC标记的细胞周期后期促进蛋白亚基10抗体

英文名称： Anti-APC10/FITC

产品编号： HZ-12483R-FITC

产品价格： null

产品产地： 中国/上海

品牌商标： HZbscience

更新时间： 2023-08-17T10:24:20

使用范围： ICC=1:50-200 IF=1:50-200

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 Rabbit Anti-APC10/FITC Conjugated antibody

FITC标记的细胞周期后期促进蛋白亚基10抗体

 

英文名称 Anti-APC10/FITC
中文名称 FITC标记的细胞周期后期促进蛋白亚基10抗体
别    名 ANAPC 10; anapc10; Anaphase promoting complex 10; Anaphase promoting complex subunit 10; Anaphase-promoting complex subunit 10; Apc 10; APC10; APC10_HUMAN; Cyclosome subunit 10; DKFZP564L0562; Doc 1; Doc1; OTTHUMP00000220297; OTTHUMP00000220298; OTTHUMP00000220299; OTTHUMP00000220300; OTTHUMP00000220303.  
规格价格 100ul/2980元 购买        大包装/询价
说 明 书 100ul  
研究领域 细胞生物  细胞周期蛋白  泛素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep, 
产品应用 ICC=1:50-200 IF=1:50-200  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 21kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human APC10
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍 background:
Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis.

Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Subunit:
The APC/C is composed of at least 12 subunits. The C-terminus of APC10 binds to CDC27/APC3. 

Similarity:
Belongs to the APC10 family.
Contains 1 DOC domain.

Database links:
UniProtKB/Swiss-Prot: Q9UM13.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
   
   

晚期促进复合物（APC）由十多个亚基组成，以细胞周期依赖的方式在有丝分裂的中期和后期过渡期间促进姐妹染色单体的分离。APC，或称环体，通过有丝分裂细胞周期素和其他调控蛋白的泛素化来完成这一进程，这些调控蛋白是细胞分裂过程中破坏的目标。APC通过蛋白激酶CDK1/cyclin B和Polo样激酶（PLK）磷酸化，从而活化。APC受到许多调节因子的严格控制，包括CDC20、CDH1和MAD2。具体地说，CDC20和CDH1直接结合并激活APCs的细胞周期泛素化活性。相反，MAD2通过与CDC20和APC形成三元复合物来抑制APC，从而防止APC激活。APC10包含DOC1同源结构域，这是与许多其他假定的E3泛素连接酶共同的β-夹心结构。APC10在整个细胞周期中与核心APC亚基结合。具体而言，APC10与CDC27／APC3的C-末端结合。在有丝分裂过程中，APC10定位于中心体和有丝分裂纺锤体中。APC10还定位于从晚期到后期的动粒，以及在末期和胞质分裂中的中心体。